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Image Search Results
Journal: PLoS ONE
Article Title: Engineering Pyranose 2-Oxidase for Modified Oxygen Reactivity
doi: 10.1371/journal.pone.0109242
Figure Lengend Snippet: Dehydrogenase activity was determined using DCPIP (light grey bars), 1,4-BQ (dark grey bars) and Fc + (mid grey bars) as electron acceptors, oxidase activity was determined by following oxygen consumption. Wild-type activities were set at 100%. D-glucose was used as electron donor at 100 mM. Results are the mean of at least duplicate independent experiments and error bars correspond to standard deviations. The diagram was prepared using SigmaPlot 11.
Article Snippet: Apparent kinetic constants were calculated by non-linear least-square regression, fitting the data to the Henri-Michaelis-Menten equation, using
Techniques: Activity Assay